This Colorimetric Hydrogen Peroxide Assay Kit uses our unique IR peroxidase substrate to quantify hydrogen peroxide in solutions and cell extracts. Upon hydrogen peroxide oxidation the colorless IR generates an intense blue color product that is pH-independent from pH 4 to 10. The existing colorimetric hydrogen peroxide assays (from other vendors) often have severe sample interferences caused by the inherent absorption of biological samples. The near infrared absorption of IR product minimizes the assay background that since the biological samples rarely absorb light beyond 600 nm. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized 'mix and read' assay that is compatible with HTS liquid handling instruments.
